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Mouse IL-5 Protein, His Tag
Catalog Number: orb762422
| Catalog Number | orb762422 |
|---|---|
| Category | Proteins |
| Description | Mouse IL-5, His Tag is expressed from human 293 cells (HEK293). It contains AA Met 21 - Gly 133 (Accession # P04401-1). |
| Target | IL-5 |
| Conjugation | Unconjugated |
| Tag | N-10×His |
| Reactivity | Mouse |
| Form/Appearance | Powder |
| Buffer/Preservatives | PBS, pH 7.4 |
| Purity | 90% |
| Protein Sequence | NP_034688.1 |
| UniProt ID | P04401 |
| MW | 15.0 KDa |
| Application notes | This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 15.0 KDa. The protein migrates as 15 kDa,19 kDa and 23-30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Expression System | HEK293 |
| Endotoxins | 1.0 EU per μg |
| Biological Origin | Mouse |
| Biological Activity | Immobilized Mouse IL-5, His Tag (Cat. No. ) at 5 μg/mL (100 μL/well) can bind Human IL-5 R alpha, Fc Tag with a linear range of 5-39 ng/mL (QC tested). |
| Expression Region | Met 21 - Gly 133 |
| Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
| Note | For research use only |
| NCBI | NP_034688.1 |
Immobilized Mouse IL-5, His Tag (Cat. No. orb762422) at 5 μg/mL (100 μL/well) can bind Human IL-5 R alpha, Fc Tag with a linear range of 5-39 ng/mL (QC tested).
Mouse IL5 Protein, His Tag [orb2698826]
> 90%, determined by SDS-PAGE
This protein contains the mouse IL5 (P04401) (Met 1-Gly 133) was expressed from Baculovirus-Insect Cells，with a C-terminal polyhistidine tag.
20 μg, 5 μgmIL5 Protein [orb1471921]
Greater than 90.0% as determined by SDS-PAGE.
Sf9, Baculovirus cells
2 μg, 10 μg, 1 mgmIL5 Protein [orb1471922]
Greater than 90.0% as determined by SDS-PAGE.
HEK293 Cells
10 μg, 1 mg, 2 μgIL-5 Protein, Mouse, Recombinant (His) [orb1957132]
99.20%
14.5 kDa (predicted); 18 kDa (reducing conditions)
20 μg, 200 μg, 500 μg, 100 μgIL-5 Protein, Mouse, Recombinant (His & Avi), Biotinylated [orb1960131]
98.00%
16.0 kDa (predicted). Due to glycosylation, the protein migrates to 16 kDa and 20-35 kDa based on Tris-Bis PAGE result.
500 μg, 100 μg, 1 mg
