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Mouse IL-5 Protein, His Tag

Catalog Number: orb762422

DispatchUsually dispatched within 5-10 working days
$ 700.00
Catalog Numberorb762422
CategoryProteins
DescriptionMouse IL-5, His Tag is expressed from human 293 cells (HEK293). It contains AA Met 21 - Gly 133 (Accession # P04401-1).
ReactivityMouse
TagN-10×His
Form/AppearancePowder
Purity90%
ConjugationUnconjugated
MW15.0 KDa
TargetIL-5
UniProt IDP04401
Protein SequenceNP_034688.1
Expression SystemHEK293
Biological OriginMouse
Biological ActivityImmobilized Mouse IL-5, His Tag (Cat. No. ) at 5 μg/mL (100 μL/well) can bind Human IL-5 R alpha, Fc Tag with a linear range of 5-39 ng/mL (QC tested).
Expression RegionMet 21 - Gly 133
Endotoxins1.0 EU per μg
NCBINP_034688.1
Storage-20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
Buffer/PreservativesPBS, pH 7.4
NoteFor research use only
Application notesThis protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 15.0 KDa. The protein migrates as 15 kDa,19 kDa and 23-30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Expiration Date6 months from date of receipt.
Mouse IL-5 Protein, His Tag

Immobilized Mouse IL-5, His Tag (Cat. No. orb762422) at 5 μg/mL (100 μL/well) can bind Human IL-5 R alpha, Fc Tag with a linear range of 5-39 ng/mL (QC tested).

  • IL-5 Protein, Mouse, Recombinant (His & Avi), Biotinylated [orb1960131]

    98.00%

    16.0 kDa (predicted). Due to glycosylation, the protein migrates to 16 kDa and 20-35 kDa based on Tris-Bis PAGE result.

    500 μg, 100 μg, 1 mg
  • IL-5 Protein, Mouse, Recombinant (His & Avi) [orb1960132]

    98.00%

    16.03 kDa (predicted). Due to glycosylation, the protein migrates to 16.03 kDa and 20-32 kDa based on Tris-Bis PAGE result.

    500 μg, 100 μg, 1 mg
  • mIL5 Protein [orb1471921]

    Greater than 90.0% as determined by SDS-PAGE.

    Sf9, Baculovirus cells

    2 μg, 10 μg, 1 mg
  • mIL5 Protein [orb1471922]

    Greater than 90.0% as determined by SDS-PAGE.

    HEK293 Cells

    10 μg, 1 mg, 2 μg
  • IL-5 Protein, Mouse, Recombinant (His) [orb1957132]

    99.20%

    14.5 kDa (predicted); 18 kDa (reducing conditions)

    20 μg, 200 μg, 500 μg, 100 μg