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Catalog Number | orb1176550 |
---|---|
Category | Proteins |
Description | Mouse IL-13, His Tag is expressed from human 293 cells (HEK293). It contains AA Ala 19 - Phe 131 (Accession # P20109-1). |
Target | IL-13 |
Conjugation | Unconjugated |
Tag | N-10×His |
Reactivity | Mouse |
Form/Appearance | Powder |
Buffer/Preservatives | PBS, pH 7.4 |
Purity | 90% |
Protein Sequence | NP_032381.1 |
UniProt ID | P20109 |
MW | 14.2 kDa |
Application notes | This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 14.2 kDa. The protein migrates as 16-30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expression System | HEK293 |
Endotoxins | 1.0 EU per μg |
Biological Origin | Mouse |
Biological Activity | Immobilized Mouse IL-13, His Tag (Cat. No. ) at 5 μg/mL (100 μL/well) can bind Mouse IL-13RA1, Fc Tag (Cat. No. IL1-M5258) with a linear range of 0.3-5 ng/mL (QC tested). |
Expression Region | Ala 19 - Phe 131 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Note | For research use only |
NCBI | NP_032381.1 |
Immobilized Mouse IL-13, His Tag (Cat. No. orb1176550) at 5 μg/mL (100 μL/well) can bind Mouse IL-13RA1, Fc Tag (Cat. No. orb257586) with a linear range of 0.3-5 ng/mL (QC tested).
> 95% by SDS-PAGE. | |
Recombinant Mouse IL-13 Protein is produced by Mammalian expression system. The target protein is expressed with sequence (Ser26-Phe131) of mouse IL-13 (Accession #P20109) fused with a 6��His Tag at the C-terminus. |
100.00% | |
15-30 KDa (reducing condition) |
100.00% | |
14-30 KDa (reducing condition) |
> 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC | |
37.55 kDa (predicted). Due to glycosylation, the protein migrates to 45-60 kDa based on Tris-Bis PAGE result. |