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Catalog Number | orb343782 |
---|---|
Category | Proteins |
Description | Mouse IgG2b isotype control Fluorescein Antibody |
Tested applications | FC |
Isotype | IgG2b |
Concentration | 1.0 mg/mL |
Dilution range | FC: 1:1000-1:5000 |
Form/Appearance | Lyophilized |
Purity | This product has been prepared from normal mouse serum by immunoaffinity chromatography using antibodies coupled to agarose beads. Extensive cross-adsorption was performed to remove any unwanted subclasses. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG2b and anti-Mouse serum. Isotyping assay resulted the material is non-reactive with antisera to mouse IgG1, IgG2a, IgG3, IgM, and IgA. Fluorescein Conjugated Mouse IgG2b was proven by Dot Blot. |
Conjugation | FITC |
Source | Mouse |
Biological Origin | Mouse |
Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | Fluorescein conjugated Mouse IgG2b isotype control Read more... |
Note | For research use only |
Application notes | Mouse IgG2b isotype control can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, Immunofluorescence, and ELISA experiments where determination of sample isotype is important. |
Expiration Date | 12 months from date of receipt. |