Mouse IgG2b isotype control Fluorescein Antibody

• Catalog Number: orb343782
• Category: Proteins
• Description: Mouse IgG2b isotype control Fluorescein Antibody
• Isotype: IgG2b
• Conjugation: FITC
• Form/Appearance: Lyophilized
• Concentration: 1.0 mg/mL
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Purity: This product has been prepared from normal mouse serum by immunoaffinity chromatography using antibodies coupled to agarose beads. Extensive cross-adsorption was performed to remove any unwanted subclasses. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG2b and anti-Mouse serum. Isotyping assay resulted the material is non-reactive with antisera to mouse IgG1, IgG2a, IgG3, IgM, and IgA. Fluorescein Conjugated Mouse IgG2b was proven by Dot Blot.
• Tested applications: FC
• Dilution range: FC: 1:1000-1:5000
• Application notes: Mouse IgG2b isotype control can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, Immunofluorescence, and ELISA experiments where determination of sample isotype is important.
• Source: Mouse
• Biological Origin: Mouse
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Alternative names: Fluorescein conjugated Mouse IgG2b isotype control
• Research Area: Protein Biochemistry
• Note: For research use only
• Expiration Date: 12 months from date of receipt.