Mouse IgG2b Isotype Control APC

• Catalog Number: orb154458
• Category: Antibodies
• Description: Mouse monoclonal antibody conjugated to APC which recognizes IgG2b that belongs to many immunoglobulin G. It can be used as a negative control, to distinguish specific from non-specific binding of mouse IgG2b fluorochrome-conjugated antibodies to human cells. It was found that IgG2a was superior to IgG1 in activating complement. IgG2 is an antibody moleculer induced by infection.
• Clonality: Monoclonal
• Clone Number: MPC-11
• Tested applications: FC
• Isotype: Mouse IgG2b kappa
• Immunogen: KLH-coupled trinitrophenol
• Antibody Type: Secondary Antibody
• Concentration: 0.1 mg/ml
• Purity: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: APC
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Note: For research use only
• Application notes: Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/ml of incubation mixture), change the conditions of your experiment to reduce the background.
• Expiration Date: 12 months from date of receipt.

Flow cytometry analysis: Example of nonspecific mouse IgG2b (MPC-11) APC signal on human peripheral blood; surface staining, 1 µg/ml.