Mouse IgG (H&L) Antibody Peroxidase Conjugated

SKU: orb346678

Description

Mouse IgG (H&L) antibody (Peroxidase)

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000
• Reactivity: Mouse
• Application Notes:
  This product is designed for ELISA and western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Mouse IgG whole molecule
• Purity: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Mouse IgG and Mouse Serum.
• Conjugation: HRP

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: None
• Concentration: 10.0 mg/mL
• Disclaimer: For research use only

Alternative Names

Rabbit anti-Mouse IgG Peroxidase Conjugated Antibody, Rb anti-Mouse IgG HRP Conjugated Antibody

In vitro characterization of the LCL scaffold and the LCL-TbpA hybrid antigens. (A) Mouse sera were evaluated in a whole cell ELISA against both wild-type N. meningitidis M982 (Black) and M982 with TbpB knocked out (M982ΔtbpB; Gray). Sera were assayed in triplicate from individual mice (4 to 5 mice per group) and averaged and displayed as mean +/- SEM. Significance was determined as a significant increase in titer compared with mice that received adjuvant alone by two-way ANOVA with Sidak's multiple comparison test for both wildtype and knockout data sets. **p ≤ 0.01, ****p ≤ 0.0001.

Whole-cell serum IgG ELISA titres of mice immunized with alum, TbpA or Hp7-L10 when captured by (A) heat-killed N. gonorrhoeae strain MS11 or (B) heat-killed N. meningitidis strain M982. Female mice were used in the lower genital tract gonococcal colonization, while male mice were challenged systemically with N. meningitidis. Significance was determined as a significant increase in titer compared with mice that received alum alone by ordinary one-way ANOVA with post-hoc analysis by Dunnett's multiple comparisons test. ****p < 0.0001.