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Catalog Number | orb1087559 |
---|---|
Category | Proteins |
Description | Mouse IGF-I, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Gly 49 - Ala 118 (Accession # P05017-1). |
Target | IGF-I |
Conjugation | Unconjugated |
Tag | N-Fc |
Reactivity | Mouse |
Form/Appearance | Powder |
Buffer/Preservatives | 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH 7.5 |
Purity | 95% |
Protein Sequence | NP_034642.2 |
UniProt ID | P05017 |
MW | 34.1 kDa |
Application notes | This protein carries a human IgG1 Fc tag at the N-terminus. The protein has a calculated MW of 34.1 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expression System | HEK293 |
Endotoxins | 1.0 EU per μg |
Biological Origin | Mouse |
Biological Activity | Immobilized Mouse IGF-I R, His Tag (Cat. No. IGR-M5223) at 5 μg/mL (100 μL/well) can bind Mouse IGF-I Protein, Fc Tag (Cat. No. ) with a linear range of 0.02-1.25 μg/mL (QC tested). |
Expression Region | Gly 49 - Ala 118 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Note | For research use only |
NCBI | NP_034642.2 |
Immobilized Mouse IGF-I R, His Tag (Cat. No. orb511248) at 5 μg/mL (100 μL/well) can bind Mouse IGF-I Protein, Fc Tag (Cat. No. orb1087559) with a linear range of 0.02-1.25 μg/mL (QC tested).
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 33.8 kDa after removal of the signal peptide. The apparent molecular mass of hFc-mIGF1 is approximately 35-55 kDa due to glycosylation. | |
Mammalian |