Mitofusin 2 Antibody: FITC

• Catalog Number: orb150814
• Category: Antibodies
• Description: Mouse monoclonal to Mitofusin 2 (FITC). Mitofusin-2 (Mfn2) is a membrane protein of the mitochondria and is important in mitochondrial fusion in mammalian cells. Mfn2 activity has also been implicated in mitochondrial metabolism and the loss of Mfn2 may be the reason for certain metabolic modifications that are seen in individuals with obesity. Mfn2 has been associated with maintaining the membrane potential of the mitochondria. Mfn2 has been shown to hinder mitochondrial antiviral signaling by inhibiting production of type 1 interferons. Mfn2 is a p53-inducible target and involved in cell proliferation, apoptosis promotion and tumor suppression..
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: N153/5 (Formerly sold as S153-5)
• Tested applications: ICC, IF, IHC
• Reactivity: Human, Mouse, Rat
• Isotype: IgG2a
• Immunogen: Fusion protein amino acids 370-600 (cytoplasmic N-terminus) of mouse Mitofusin-2. Rat: 97% identity (226/231 amino acids identical). Human: 92% identity (214/231 amino acids identical) ~55% identity with Mitofusin-1.
• Concentration: 1 mg/ml
• Dilution range: WB (1:1000), ICC/IF (1:100)
• Conjugation: FITC
• MW: 90kDa
• Target: Mitofusin 2
• Entrez: 170731
• UniProt ID: Q80U63
• NCBI: NP_573464.2
• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 640.91mM DMSO, 136.36mM Ethanolamine, 9.09mM Sodium Bicarbonate in 90.9% PBS
• Alternative names: CMT2A antibody, CMT2A2 antibody, CPRP 1 antibody,
• Note: For research use only
• Application notes: 1 µg/ml of SMC-457 was sufficient for detection of Mitofusin-2 in 20 µg of mouse cardiac mitochondrial lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Mitofusin 2 Monoclonal Antibody, Clone N153/5. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Mitofusin 2 Monoclonal Antibody at 1:100 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Mitofusin 2 Antibody (D) Composite.