MITF Antibody (N-term)

• Catalog Number: orb1938110
• Category: Antibodies
• Description: MITF Antibody (N-term)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Mouse, Rat
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: O75030
• MW: 58795 Da
• Tested applications: FC, IF, WB
• Dilution range: WB - 1:1000, IF - 1:10-50, FC - 1:10-50, IHC - 1:800
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: BHLHE32
• Research Area: Immunology & Inflammation
• Note: For research use only

Anti-MITF Antibody (N-term) at 1:2000 dilution + A375 whole cell lysates.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 59 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Confocal immunofluorescent analysis of MITF Antibody (N-term) with hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

Immunohistochemical analysis of paraffin-embedded Human Melanoma section using Pink1. diluted at 1:800 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

MITF Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

MITF Antibody (N-term) western blot analysis in K562 cell line lysates (35 ug/lane). This demonstrates the MITF antibody detected the MITF protein (arrow).