Mib1/Mindbomb Antibody (N-term)

• Catalog Number: orb1931665
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB4448
• Tested applications: IF, IHC-P, WB
• Predicted Reactivity: Mouse, Other, Zebrafish
• Reactivity: Human
• Isotype: Rabbit IgG
• Dilution range: IF: 1:20~100, WB: 1:1000, IHC-P: 1:50~100
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 110136 Da
• Target: This Mib1/Mindbomb antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13-42 amino acids from the N-terminal region of human Mib1/Mindbomb.
• UniProt ID: Q86YT6
• NCBI: NP_065825.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: E3 ubiquitin-protein ligase MIB1, 632-, DAPK-inter
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescent staining of Hela cells incubated with MIB Antibody. (N-term) at a dilution of 1: 20.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, .which was peroxidase-conjugated to the secondary antibody, followed by AEC.staining. This data demonstrates the use of this antibody for.immunohistochemistry; clinical relevance has not been evaluated. BC = breast.carcinoma; HC = hepatocarcinoma.

MIB Antibody (K28) western blot analysis in K562 cell line lysates (35 ug/lane). This demonstrates the MIB antibody detected the MIB protein (arrow).