Mesothelin Antibody / MSLN

• Catalog Number: orb534119
• Category: Antibodies
• Description: Mesothelin is a 40kDa glycosyl-phosphatidylinositol-anchored glycoprotein cleaved from a 69kDa precursor protein. Mesothelin immunoreactivity is high in cancers of the ovary (serous papillary, endometrioid and undifferentiated) and pancreas, with less frequent staining seen in adenocarcinomas of the endometrium, lung and stomach/esophagus. Mesothelin is one of the most sensitive markers for mesothelioma.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG2b, kappa
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Immunogen: A portion of amino acids 273-407 from the human protein was used as the immunogen for this MSLN antibody.
• UniProt ID: Q13421
• Tested applications: IHC-P
• Dilution range: IHC (FFPE): 1-2ug/ml for 30 min at RT
• Application notes: Optimal dilution of the MSLN antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: MKPF-1
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This MSLN antibody is available for research use only.
• Note: For research use only

IHC testing of FFPE human lung mesothelioma with MSLN antibody (clone MKPF-1). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE rat stomach with MSLN antibody (clone MKPF-1). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.