MDH2

• Catalog Number: orb389375
• Category: Antibodies
• Description: Goat polyclonal antibody to MDH2
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Bovine, Human, Mouse, Porcine, Rat
• Dilution range: ELISA: 1:8000, WB: 0.01-0.03 μg/ml, IHC-P: 5 μg/ml
• Conjugation: Unconjugated
• MW: 35.5
• Target: MDH2
• Entrez: 4191
• Protein Sequence: TRDDLFNTNAT
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: MDH2, malate dehydrogenase 2, NAD (mitochondrial),
• Note: For research use only
• Application notes: WB: Approx 35kDa band observed in lysates of cell lines HeLa, HepG2 and NIH3T3 (calculated MW of 35.5kDa according to Human NP_005909.2 and 35.6kDa according to Mouse NP_032643.2 ).
• Expiration Date: 12 months from date of receipt.

0.1 µg/ml staining of HeLa (A), HepG2 (B) and NIH3T3 (C) lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.

0.01 µg/ml staining of Human (A), Mouse (B), Rat (C) and Pig (D) Heart lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.

5 µg/ml staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.