Mca-RPPGFSAFK(Dnp)-OH

• Catalog Number: orb1140573
• Category: Proteins
• Description: FRET substrate for endothelin-converting enzyme-1 (ECE-1); Fluorescent labelled peptides.
• CAS Number: 323577-36-2
• Modifications: 7-methoxycoumarin (Mca) at the N terminal, Lysine 9 has 2, 4-dinitrophenyl (Dnp) attached.
• Structure: Mca-Arg-Pro-Pro-Gly-Phe-Ser-Ala-Phe-Lys-(Dnp)
• Target Areas: Endothelin converting enzyme 1
• Form/Appearance: Freeze dried solid
• Purity: > 95% by HPLC
• MW: 1388.5 Da
• Formula: C₆₆H₈₁N₁₅O₁₉
• Solubility (25°C): Soluble in DMSO
• Protein Sequence: Mca-Arg-Pro-Pro-Gly-Phe-Ser-Ala-Phe-Lys-(Dnp)
• Storage: Store desiccated, frozen and in the dark
• Alternative names: 2,4-dinitrophenyl, 323577-36-2, Dnp) group, ECE-1,
• Background: Mca-RPPGFSAFK(Dnp)-OH, also known as Mca-(Ala7,Lys(Dnp)9)-bradykinin is a very sensitive fluorescent substrate for endothelin-converting enzyme-1 (ECE-1), the membrane-bound zinc metallopeptidase that is related to neprilysin. Mca-RPPGFSAFK(Dnp)-OH can also be used for assaying ACE1, ACE2, BACE, cathepsin A, cathepsin X/Z, ECE-1, ECE-2, MMP-2, MMP9, neprilysin, insulinase and thermolysin (TLN), although it is not a substrate for MMP1. Mca-RPPGFSAFK(Dnp)-OH substrate contains a highly fluorescent 7-methoxycoumarin (Mca) group that is efficiently quenched by resonance energy transfer to the 2,4-dinitrophenyl (Dnp) group.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.