LY75/DEC-205 Antibody / CD205

• Catalog Number: orb2635008
• Category: Antibodies
• Description: DEC-205 (LY75, lymphocyte antigen 75, GP200-MR6) is a 1,695 residue (mature form) multi-lectin receptor that belongs to the MMR (macrophage mannose receptor) family of multidomain molecules. MMR family molecules mediate membrane receptor targeting to endosomes or lysosomes rich in major histocompatibility complex class II (MHC II) products. Expressed in mature dendritic cells (DC), DEC-205 contains an extracellular N-terminal cysteine-rich domain, a fibronectin type II domain, ten C-type carbohydrate recognition domains, a single transmembrane region and a small cytoplasmic C-terminal domain (31 amino acids) containing a tyrosine at 1679. DEC-205 elicits either an agonistic or antagonistic effect on IL-4 function, which is demonstrated by the ability of DEC-205 to imitate IL-4-induced maturation of epithelium or to inhibit IL-4-induced proliferation of T cells, respectively. Acts as an endocytic receptor to direct captured antigens from the extracellular space to a specialized antigen-processing compartment (By similarity). Causes reduced proliferation of B-lymphocytes.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CD205/3720
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG2b, kappa
• Immunogen: A portion of amino acids 1006-1130 was used as the immunogen for the LY75/DEC-205 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This LY75/DEC-205 antibody is available for research use only.
• UniProt ID: O60449
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Application notes: Optimal dilution of the LY75/DEC-205 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human colon tissue with LY75/DEC-205 antibody (clone CD205/3720). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human kidney tissue with LY75/DEC-205 antibody (clone CD205/3720). Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free LY75/DEC-205 antibody (clone CD205/3720) as confirmation of integrity and purity.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using LY75/DEC-205 antibody (clone CD205/3720). These results demonstrate the foremost specificity of the CD205/3720 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.