Ly6A Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb1172792
• Category: Antibodies
• Description: Ly6A Recombinant Rabbit Monoclonal Antibody
• Target: Ly6a
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Mouse
• Predicted Reactivity: Mouse
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Clone Number: E12A2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Research Area: Developmental Biology, Hematopoietic Stem Cell Mar
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:2000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: 1: C2C12 cell lysate, the Ly6A Rabbit mAb pre-adsorbed with 3μM of the synthetic peptides, 2: C2C12 cell lysate, the Ly6A Rabbit mAb with no peptide blocking. Protein loading quantity: 20 µg, Exposure time: 10 s, Predicted MW: 14 kDa, Observed MW: 13 kDa.

Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:2000, Primary ab incubation condition: 2 hours at room temperature, Secondary ab: Goat Anti-Rabbit IgG H&L (HRP), Lysate: 1: Mouse thymus, 2: C2C12, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 14 kDa, Observed MW: 13 kDa.

Tissue: Mouse testis, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:100, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1172792.