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Catalog Number | orb750304 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody against KRT7 conjugated to Biotin |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | OV-TL12/30 |
Tested applications | FACS, IF, IHC-P, WB |
Reactivity | Human |
Isotype | Mouse IgG1, kappa |
Immunogen | The ovarian carcinoma cell line OTN 11 was used as the immunogen for the Cytokeratin 7 antibody. |
Antibody Type | Primary Antibody |
Dilution range | Flow cytometry: 5ul per test per one million cells or 5ul per 100ul of whole blood,Immunofluorescence: 2-4ug/ml,Western blot: 2-4ug/ml,Immunohistochemistry (FFPE): 2-4ug/ml at RT |
Purity | Protein G affinity chromatography |
Conjugation | Biotin |
Formula | 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
Hazard Information | This Cytokeratin 7 antibody is available for research use only. |
UniProt ID | P08729 |
Storage | Store the Cytokeratin 7 antibody at 2-8°C (up to one month) or aliquot and store at -20°C (longer term). |
Buffer/Preservatives | 0.1 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide |
Note | For research use only |
Application notes | Optimal dilution of the Cytokeratin 7 antibody should be determined by the researcher. |
Expiration Date | 12 months from date of receipt. |
IHC staining of FFPE human prostate carcinoma with Cytokeratin 7 antibody (clone OV-TL12/30). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human prostate carcinoma with Cytokeratin 7 antibody (clone OV-TL12/30). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free Cytokeratin 7 antibody (clone OV-TL12/30) as confirmation of integrity and purity.
FACS, IF, IHC-P, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
FACS, IF, IHC-P, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |