KRT7 Antibody / Cytokeratin 7

• Catalog Number: orb749623
• Category: Antibodies
• Description: It recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This mAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This mAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: A semi-purified cytokeratin preparation was used as the immunogen for the Keratin 7 antibody.
• UniProt ID: P08729
• Tested applications: FACS, IF, IHC-P, WB
• Dilution range: Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 0.5-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
• Application notes: Optimal dilution of the Keratin 7 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Antibody Type: Primary Antibody
• Clone Number: K72.7
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This Keratin 7 antibody is available for research use only.
• Note: For research use only

IHC: Formalin-fixed, paraffin-embedded human lung SCC stained with Keratin 7 antibody (clone K72.7).

IHC: Formalin-fixed, paraffin-embedded ovarian carcinoma stained with Keratin 7 antibody (clone K72.7).

Immunofluorescent staining of methanol-fixed human HeLa cells with Cytokeratin 7 antibody (green, clone K72.7) and Reddot nuclear stain (red).

Western blot testing of human HeLa cell lysate with Cytokeratin 7 antibody (clone K72.7).

Flow cytometry testing of PFA-fixed human HeLa cells with Cytokeratin 7 antibody (clone K72.7); Red = isotype control, Blue = recombinant Cytokeratin 7 antibody.