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Catalog Number | orb1929871 |
---|---|
Category | Antibodies |
Description | Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB18413 |
Tested applications | FC, IHC-P, WB |
Predicted Reactivity | Rat |
Reactivity | Human |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Conjugation | Unconjugated |
MW | 58827 Da |
Target | This KRT10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 318-346 amino acids from the Central region of human KRT10. |
UniProt ID | P13645 |
NCBI | NP_000412.3 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Keratin, type I cytoskeletal 10, Cytokeratin-10, C Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Formalin-fixed and paraffin-embedded human skin reacted with KRT10 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of KRT10 antibody (Center) in CEM cell line lysates (35 ug/lane). KRT10 (arrow) was detected using the purified Pab.
KRT10 Antibody (Center) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.