KRAS Antibody (C-term)

• Catalog Number: orb1934944
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB32708
• Tested applications: FC, IF, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: IF: 1:10~50, WB: 1:500, WB: 1:2000, WB: 1:2000, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 21656 Da
• Target: This KRAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 146-174 amino acids from the C-terminal region of human KRAS.
• UniProt ID: P01116
• NCBI: NP_203524.1, NP_004976.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: GTPase KRas, K-Ras 2, Ki-Ras, c-K-ras, c-Ki-ras, G
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

KRAS Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Anti-KRAS Antibody (C-term) at 1:2000 dilution + HT-29 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 22 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Confocal immunofluorescent analysis of KRAS Antibody (C-term) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

All lanes: Anti-KRAS Antibody (C-term) at 1:500 dilution. Lane 1: Hela whole cell lysate. Lane 2: 293T/17 whole cell lysate. Lane 3: C2C12 whole cell lysate. Lane 4: C6 whole cell lysate. Lane 5: HT-29 whole cell lysate. Lane 6: PC-12 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 22 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-KRAS Antibody (C-term) at 1:2000 dilution. Lane 1: 293T/17 whole cell lysate. Lane 2: C2C12 whole cell lysate. Lane 3: Hela whole cell lysate. Lane 4: HT-29 whole cell lysate. Lane 5: K562 whole cell lysate. Lane 5: PC-12 whole cell lysate. Lane 5: Ramos whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 22 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.