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Item 1 of 5
Item 1 of 5
IRF6 Recombinant Rabbit Monoclonal Antibody
Catalog Number: orb559090
| Catalog Number | orb559090 |
|---|---|
| Category | Antibodies |
| Description | IRF6 Recombinant Rabbit Monoclonal Antibody |
| Target | IRF6 |
| Clonality | Recombinant |
| Species/Host | Rabbit |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Form/Appearance | Liquid |
| Concentration | 1mg/ml |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Immunogen | Recombinant human IRF6 protein, around C-terminal 150aa |
| UniProt ID | O14896 |
| MW | 53 kDa |
| Tested applications | ICC, IF, IHC-Fr, IHC-P, WB |
| Dilution range | WB=1:1000-2000, IHC-P=1:50-200, IHC-F=1:50-200, ICC/IF=1:50-200, IF=1:50-200 |
| Antibody Type | Recombinant Antibody |
| Clone Number | 1A1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Interferon regulatory factor 6; IRF 6; IRF-6; IRF6 Read more... |
| Note | For research use only |
Paraformaldehyde-fixed, paraffin embedded (human breast), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (IRF6) Monoclonal Antibody, Unconjugated (orb559090) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (IRF6) Monoclonal Antibody, Unconjugated (orb559090) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (IRF6) Monoclonal Antibody, Unconjugated (orb559090) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human skin), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (IRF6) Monoclonal Antibody, Unconjugated (orb559090) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Lane 1: Human K562 cell lysates, Lane 2: Human MCF-7 cell lysates, Lane 3: Human SW480 cell lysates, Lane 4: Human A431 cell lysates, Lane 5: Human SH-SY5Y cell lysates, Primary: Anti-IRF6 (orb559090) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 53 kDa, Observed band size: 58 kDa.
