ID1 Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb704400
• Category: Antibodies
• Description: ID1 Recombinant Rabbit Monoclonal Antibody
• Target: ID1
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: Recombinant human ID1 protein
• UniProt ID: P41134
• MW: 16 kDa
• Tested applications: FC, ICC, IF, IHC-Fr, IHC-P
• Dilution range: ICC/IF=1:50-200, Flow-Cyt=1:50-100, IHC-P=1:50-200, IHC-F=1:50-200, IF=1:50-200
• Antibody Type: Primary Antibody
• Clone Number: 5F1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: bHLHb24; Class B basic helix-loop-helix protein 24
• Research Area: Angiogenesis, Cancer Research, Epigenetics and Nuc
• Note: For research use only

Sample: Lane 1: HepG2 (Human) Cell Lysate at 30 ug, Lane 2: MCF-7 (Human) Cell Lysate at 30 ug, Lane 3: U-2os (Human) Cell Lysate at 30 ug, Lane 4: A549 (Human) Cell Lysate at 30 ug, Lane 5: Hela (Human) Cell Lysate at 30 ug, Primary: Anti-ID1 (orb704400) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 16 kD, Observed band size: 25 kD.

U2OS cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (ID1) monoclonal Antibody, Unconjugated (orb704400) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.