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Catalog Number | orb244902 |
---|---|
Category | Proteins |
Description | Recombinant human Ubiquitin-conjugating enzyme E2 W |
Tag | N-terminal 6xHis-SUMO-tagged |
Form/Appearance | Liquid or Lyophilized powder |
Purity | Greater than 90% as determined by SDS-PAGE. |
MW | 33.3 kDa |
UniProt ID | Q96B02 |
Protein Sequence | MASMQKRLQKELLALQNDPPPGMTLNEKSVQNSITQWIVDMEGAPGTLYEGEKFQLLFKFSSRYPFDSPQVMFTGENIPVHPHVYSNGHICLSILTEDWSPALSVQSVCLSIISMLSSCKEKRRPPDNSFYVRTCNKNPKKTKWWYHDDTC |
Protein Length | Full Length |
Source | E.coli |
Biological Origin | Homo sapiens (Human) |
Expression Region | 1-151aa |
Endotoxins | Not test. |
Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃. |
Buffer/Preservatives | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
Alternative names | UBE2W Read more... |
Note | For research use only |
Application notes | This is His-SUMO-tag protein |
Expiration Date | 6 months from date of receipt. |
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Greater than 90.0% as determined by SDS-PAGE. | |
Escherichia Coli |
98.00% | |
19.2 kDa (predicted); 18 kDa (reducing conditions) |
ELISA, WB | |
Greater than 95% as determined by SDS-PAGE | |
17.7 kDa | |
E.Coli |
> 80%, determined by SDS-PAGE | |
This protein contains the mature form of human UBE2A (P49459) (Met 1-Cys 152) was expressed from E. coli. |