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Catalog Number | orb1149290 |
---|---|
Category | Proteins |
Description | Human TNFSF11 Protein, premium grade is expressed from human 293 cells (HEK293). It contains AA Gly 64 - Asp 245 (Accession # AAC51762.1). |
Target | TNFSF11 |
Conjugation | Unconjugated |
Tag | Native |
Reactivity | Human |
Form/Appearance | Powder |
Buffer/Preservatives | PBS, pH 7.4 |
Purity | 95% |
Protein Sequence | AAC51762.1 |
MW | 20.5 kDa |
Application notes | This protein carries no "tag". The protein has a calculated MW of 20.5 kDa. The protein migrates as 28-33 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expression System | HEK293 |
Endotoxins | 0.1 EU per μg |
Biological Origin | Human |
Biological Activity | Immobilized Human TNFSF11 Protein, premium grade (Cat. No. ) at 2 μg/mL (100 μL/well) can bind Human RANK, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. RAK-H5251) with a linear range of 0.1-4 ng/mL (QC tested). |
Expression Region | Gly 64 - Asp 245 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Note | For research use only |
NCBI | AAC51762.1 |
Immobilized Human TNFSF11 Protein, premium grade (Cat. No. orb1149290) at 2 μg/mL (100 μL/well) can bind Human RANK, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. orb511285) with a linear range of 0.1-4 ng/mL (QC tested).
Greater than 90% as determined by SDS-PAGE. | |
22.4 kDa | |
E.coli |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 48.4 kDa after removal of the signal peptide.The apparent molecular mass of hFc-TNFSF11 is approximately 55-70 kDa due to glycosylation. | |
Mammalian |
> 90% as determined by SDS-PAGE. | |
48.32 kDa |
> 90% as determined by SDS-PAGE. | |
30.67 kDa |
> 95%, determined by SDS-PAGE | |
This protein contains the human TNFSF11 (O14788-2) (Gly 63-Asp 244) was expressed from HEK293 Cells. |