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Catalog Number | orb427834 |
---|---|
Category | Proteins |
Description | Recombinant of human PRDX5 protein |
Form/Appearance | Sterile Filtered colorless solution. |
Buffer/Preservatives | The PRDX5 solution contains 20mM HEPES buffer (pH 7.4). |
Purity | Greater than 95.0% as determined by SDS-PAGE. |
Protein Sequence | MAPIKVGDAI PAVEVFEGEP GNKVNLAELF KGKKGVLFGV PGAFTPGCSK THLPGFVEQA EALKAKGVQV VACLSVNDAF VTGEWGRAHK AEGKVRLLAD PTGAFGKETD LLLDDSLVSI FGNRRLKRFS MVVQDGIVKA LNVEPDGTGL TCSLAPNIIS QL |
Application notes | Enzymes |
Source | Escherichia Coli |
Biological Activity | The specific activity was found to be approximately 117-136 pmole/min/µg. The enzymatic activity was confirmed by measuring the remaining peroxide after incubation of PRDX5 and peroxide for 20 min at room temperature. Specific activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25°C for 1 minute. |
Storage | Stability: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles |
Alternative names | Peroxiredoxin-5 mitochondrial, Prx-V, Peroxisomal Read more... |
Note | For research use only |
Human | |
12.5 pg/mL-800 pg/mL | |
3.12 pg/mL |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
Human cells |
> 90% as determined by SDS-PAGE. | |
18.79 kDa |
ELISA, WB | |
Greater than 95% as determined by SDS-PAGE | |
17.7 kDa | |
E.Coli |
> 95%, determined by SDS-PAGE | |
This protein contains the human PRDX5 (isoform cytoplasmic + peroxisomal) (P30044-2) (Met 53-Leu 214) was expressed with a polyhistidine tag at the N-terminus and expressed from E. coli. |