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Item 1 of 3
Human PDGF-BB Protein, His-Avi Tag
Catalog Number: orb334919
| Catalog Number | orb334919 |
|---|---|
| Category | Proteins |
| Description | Unconjugated Human PDGF-BB, His, is expressed from E. coli cells. It contains AA Ser 82 - Thr 190 (Accession # P01127-1). |
| Target | PDGF-BB |
| Conjugation | Unconjugated |
| Tag | C-6×His & C-Avi |
| Reactivity | Human |
| Form/Appearance | Powder |
| Buffer/Preservatives | 0.085% TFA in 30% ACN |
| Purity | 95% |
| Protein Sequence | NP_002599.1 |
| UniProt ID | P01127 |
| MW | 15.1 kDa |
| Application notes | This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag . The protein has a calculated MW of 15.1 kDa. the protein migrates as 17 kDa under reducing (R) condition, and 35 kDa under non-reducing (NR) condition (SDS-PAGE). |
| Expression System | E. coli |
| Endotoxins | 1.0 EU per μg |
| Biological Origin | Human |
| Biological Activity | Immobilized Unconjugated Human PDGF-BB, His, (Cat. No. ) at 1 μg/mL (100 μL/well) can bind Human PDGF R beta, Fc Tag (Cat. No. PDB-H5259) with a linear range of 0.5-16 ng/mL (QC tested). |
| Expression Region | Ser 82 - Thr 190 |
| Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
| Note | For research use only |
| NCBI | NP_002599.1 |
SDS-PAGE analysis of Human PDGF-B protein
Line graph illustrates about the sensitivity range of Human PDGF-B protein using different concentrations
Immobilized Unconjugated Human PDGF-BB, His, Avitag (Cat. No. orb334919) at 1 μg/mL (100 μL/well) can bind Human PDGF R beta, Fc Tag (Cat. No. orb1146967) with a linear range of 0.5-16 ng/mL (QC tested).
