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Catalog Number | orb392148 |
---|---|
Category | Proteins |
Description | Recombinant human MMP3 protein is produced by our Mammalian expression system and the target gene encoding Tyr18-Cys477 is expressed with a 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Liquid in a 0.2 μM filtered solution of 20mM Tris-HCl, 150mM NaCl, 0.05% Brij35, 10% Glycerol, pH 7.5. |
Purity | Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. |
Target | MMP3 |
Entrez | 4314 |
UniProt ID | P08254 |
Protein Sequence | YPLDGAARGE DTSMNLVQKY LENYYDLEKD VKQFVRRKDS GPVVKKIREM QKFLGLEVTG KLDSDTLEVM RKPRCGVPDV GHFRTFPGIP KWRKTHLTYR IVNYTPDLPK DAVDSAVEKA LKVWEEVTPL TFSRLYEGEA DIMISFAVRE HGDFYPFDGP GNVLAHAYAP GPGINGDAHF DDDEQWTKDT TGTNLFLVAA HEIGHSLGLF HSANTEALMY PLYHSLTDLT RFRLSQDDIN GIQSLYGPPP DSPETPLVPT EPVPPEPGTP ANCDPALSFD AVSTLRGEIL IFKDRHFWRK SLRKLEPELH LISSFWPSLP SGVDAAYEVT SKDLVFIFKG NQFWAIRGNE VRAGYPRGIH TLGFPPTVRK IDAAISDKEK NKTYFFVEDK YWRFDEKRNS MEPGFPKQIA EDFPGIDSKI DAVFEEFGFF YFFTGSSQLE FDPNAKKVTH TLKSNSWLNC VDHHHHHH |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | Liquid in a 0.2 μM filtered solution of 20mM Tris-HCl, 150mM NaCl, 0.05% Brij35, 10% Glycerol, pH 7.5. |
Alternative names | STMY1; Stromelysin-1; SL-1; Matrix metalloproteina Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
Greater than 90% as determined by SDS-PAGE. | |
58.5 kDa | |
E.coli |
> 95% as determined by SDS-PAGE | |
60 kDa |
97.30% | |
50-65 KDa (reducing condition) |