Human Methotrexate (MTX) ELISA Kit

• Catalog Number: orb782443
• Category: Assays and Kits
• Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Methotrexate(MTX) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Methotrexate(MTX).
• Reactivity: Human
• Concentration: 1000 ng/mL
• Tested applications: ELISA
• Application notes: standard: 1000 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human MTX protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MTX. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MTX in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Competitive
• Assay Time: 2.5h
• Range: 15.63-1000 ng/mL
• Sensitivity: 4.57 ng/mL
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Alternative names: Methotrexate ELISA Kit, Amethopterin ELISA Kit
• Research Area: Cancer Biology
• Note: For research use only