Human Meprin A Alpha (MEP1a) ELISA Kit

• Catalog Number: orb779225
• Category: Assays and Kits
• Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Meprin A Alpha(MEP1a) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Meprin A Alpha(MEP1a).
• Reactivity: Human
• Concentration: 4000 pg/mL
• UniProt ID: Q16819
• Tested applications: ELISA
• Application notes: standard: 4000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human MEP1a protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MEP1a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MEP1a in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Competitive
• Assay Time: 2.5h
• Range: 62.5-4000 pg/mL
• Sensitivity: 17 pg/mL
• Sample Types: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Alternative names: PPHA ELISA Kit, PABA Peptide Hydrolase ELISA Kit,
• Note: For research use only