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| Catalog Number | orb2667707 |
|---|---|
| Category | Assays and Kits |
| Description | Human Kallikrein 1 (KLK1) ELISA Kit |
| UniProt ID | P06870 |
| Application notes | standard: 20 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human KLK1 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KLK1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KLK1 in the samples is then determined by comparing the OD of the samples to the standard curve |
| Assay Type | Competitive |
| Assay Time | 2.5h |
| Range | 0.32-20 ng/mL |
| Sensitivity | 0.12 ng/mL |
| Sample Types | Serum, plasma and other biological fluids |
| Alternative names | KLKR; Klk6; HK1; Kallikrein 1,Renal/Pancreas/Saliv Read more... |
| Research Area | Cancer Biology, Gastroenterology & Hepatology, Pro Read more... |
| Note | For research use only |
| Expiration Date | Please enquire. |
Human | |
0.78 ng/mL-50 ng/mL | |
0.195 ng/mL |
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