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| Catalog Number | orb779226 |
|---|---|
| Category | Assays and Kits |
| Description | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Ischemia Modified Albumin(IMA) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Ischemia Modified Albumin(IMA). |
| Reactivity | Human |
| Concentration | 30000 ng/mL |
| Tested applications | ELISA |
| Application notes | standard: 30000 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human IMA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IMA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IMA in the samples is then determined by comparing the OD of the samples to the standard curve |
| Assay Type | Competitive |
| Assay Time | 2.5h |
| Range | 468.75-30000 ng/mL |
| Sensitivity | 156.9 ng/mL |
| Sample Types | serum, plasma and other biological fluids |
| Alternative names | IMA |
| Note | For research use only |
Human IMA ELISA Kit [orb406966]
Human
3.12 IU/mL-200 IU/mL
0.78 IU/mL
24 T, 10 x 96 T, 5 x 96 T, 48 T, 96 T- Item 1 of 1
Human IMA (Ischemia Modified Albumin) ELISA Kit [orb2648121]
Human
7.813-500ng/ml
4.688ng/ml
96 T, 48 T
