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Human IL5 Protein, His Tag
SKU: orb689428
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Description
Images & Validation
−Item 1 of 4
Key Properties
−| Source | Mammalian |
|---|---|
| Target | IL5 |
| Reactivity | Human |
| Tag | C-6×His Tag |
| Molecular Weight | The protein has a predicted molecular mass of 13.94 kDa after removal of the signal peptide.The apparent molecular mass of IL5-His is approximately 15-25 kDa due to glycosylation. |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| Purity | The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
Storage & Handling
−| Storage | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
|---|---|
| Form/Appearance | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Buffer/Preservatives | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. |
| Disclaimer | For research use only |
Alternative Names
−IL-5;TRF;Interleukin-5;EDF
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Human CD30 Protein, His Tag on SDS-PAGE under reducing condition.
ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human CD30, His tagged protein (orb689465) can bind Anti-CD30 Neutralizing antibody orb689322 in a linear range of 0.13-80.0 ng/ml.
ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human CD30, His tagged protein (orb689465) can bind Human CD30 Ligand, mFc-His tagged protein orb689400 in a linear range of 2.56-320 ng/ml.
Flow cytometry analysis with 1 μg/mL Human CD30 Protein, His tag (orb689465) on Expi293 cells transfected with human CD30L (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
Quick Database Links
Gene Symbol
IL5
UniProt
UniProt Details
− No UniProt data available
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Protocol Information
Human IL5 Protein, His Tag (orb689428)
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