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Human IL10 protein
Catalog Number: orb80067
| Catalog Number | orb80067 |
|---|---|
| Category | Proteins |
| Description | IL10 Human Recombinant produced in E.coli is non covalent dimer of polypeptide chains containing 161 amino acids each and having a molecular mass of 18606.29 Dalton.The IL-10 is purified by proprietary chromatographic techniques. |
| Tested applications | FA, HPLC, SDS-PAGE |
| Purity | > 95.0% as determined by RP-HPLC and analysis by SDS-PAGE |
| Conjugation | Unconjugated |
| Target | IL10 |
| Solubility (25°C) | It is recommended to reconstitute the lyophilized Interleukin-10 in sterile H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
| Protein Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Pro-Gly-Gln-Thr. |
| Biological Origin | E.coli |
| Biological Activity | The ED50 as determined by the dose-dependant stimulation of mouse MC-9 cells was found to be 0.5 ng/ml, corresponding to Specific Activity of 3,000,000 IU/mg. |
| Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
| Buffer/Preservatives | The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Pro-Gly-Gln-Thr.. The protein was lyophilized from concentrated (1mg/ml) solution containing no additives. |
| Alternative names | B-TCGF protein, CSIF protein, TGIF protein, IL-10 Read more... |
| Note | For research use only |
| Application notes | < strong>IHC: E.coli |
| Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human IL10 protein
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Human IL10 protein (Active) [orb358975]
> 95% as determined by SDS-PAGE and HPLC.
18.6 kDa
E.Coli
500 μg, 10 μg, 100 μg - Item 1 of 1
- Item 1 of 1
Human IL10 Protein, His Tag [orb1471796]
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 19.5 kDa after removal of the signal peptide. The apparent molecular mass of His-IL10 is approximately 15-25 kDa due to glycosylation.
Mammalian
10 μg, 50 μg, 100 μg - Item 1 of 1
Human IL10 Protein, hFc Tag [orb1291070]
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 44.8 kDa after removal of the signal peptide. The apparent molecular mass of hFc-IL10 is approximately 35-55 kDa due to glycosylation.
Mammalian
100 μg, 10 μg, 50 μg 
Human IL10 protein [orb391908]
ELISA, MS, SDS-PAGE, WB
Greater than 95% as determined by reducing SDS-PAGE.
Human cells
500 μg, 50 μg, 10 μg
