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Catalog Number | orb612145 |
---|---|
Category | Proteins |
Description | Human IL-33 Protein, His Tag, premium grade is expressed from human 293 cells (HEK293). It contains AA His 109 - Thr 270 (Accession # O95760-1). |
Target | IL-33 |
Conjugation | Unconjugated |
Tag | N-10×His |
Reactivity | Human |
Form/Appearance | Powder |
Buffer/Preservatives | PBS, pH 7.4 |
Purity | 95% |
Protein Sequence | AAH47085.1 |
UniProt ID | O95760 |
MW | 20.2 kDa |
Application notes | This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 20.2 kDa. The protein migrates as 25-30 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expression System | HEK293 |
Endotoxins | 0.1 EU per μg |
Biological Origin | Human |
Biological Activity | Loaded Human IL-1RL1, Fc Tag (Cat. No. IL1-H5250) on Protein A Biosensor, can bind with Human IL-33 Protein, His Tag, premium grade (Cat. No. ) an affinity constant of 1.31 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). |
Expression Region | His 109 - Thr 270 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Note | For research use only |
NCBI | AAH47085.1 |
Loaded Human IL-1RL1, Fc Tag (Cat. No. orb257583) on Protein A Biosensor, can bind with Human IL-33 Protein, His Tag, premium grade (Cat. No. orb612145) an affinity constant of 1.31 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Greater than 90.0% as determined by SDS-PAGE. | |
Escherichia Coli |