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Human IL-3 Protein, His Tag
Catalog Number: orb1087548
| Catalog Number | orb1087548 |
|---|---|
| Category | Proteins |
| Description | Human IL-3 Protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Ala 20 - Phe 152 (Accession # P08700-1). |
| Target | IL-3 |
| Conjugation | Unconjugated |
| Tag | C-10×His |
| Reactivity | Human |
| Form/Appearance | Powder |
| Buffer/Preservatives | PBS, pH 7.4 |
| Purity | 90% |
| Protein Sequence | NP_000579.2 |
| UniProt ID | P08700 |
| MW | 17.0 kDa |
| Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 17.0 kDa. The protein migrates as 25-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Expression System | HEK293 |
| Endotoxins | 0.3 EU per μg |
| Biological Origin | Human |
| Biological Activity | Loaded Human IL-3 R alpha, Fc Tag (Cat. No. ILA-H5252) on Protein A Biosensor, can bind Human IL-3 Protein, His Tag, premium grade (Cat. No. ) with an affinity constant of 0.71 μM as determined in BLI assay (ForteBio Octet Red96e) (QC tested). |
| Expression Region | Ala 20 - Phe 152 |
| Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
| Note | For research use only |
| NCBI | NP_000579.2 |
Loaded Human IL-3 R alpha, Fc Tag on Protein A Biosensor, can bind Human IL-3 Protein, His Tag, premium grade (Cat. No. orb1087548) with an affinity constant of 0.71 μM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
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Human CD123 Protein, hFc-His Tag [orb689364]
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 80-90 kDa after removal of the signal peptide.
Mammalian
10 μg, 50 μg, 100 μg 
Human IL3 Protein, His Tag [orb2697477]
> 95%, determined by SDS-PAGE
This protein contains the human IL3 (NP_0005792) (Met 1-Phe 152) was fused with a polyhistidine tag at the C-terminus and expressed from Baculovirus-Insect Cells.
1 mg, 50 μgHuman IL3 Protein, His Tag [orb2700569]
> 95%, determined by SDS-PAGE
This protein contains the human IL3 (NP_000579.2) (Met1-Phe152) was expressed with a polyhistidine tag at the C-terminus and expressed from HEK293 Cells.
10 μg, 5 μgRecombinant Human IL-3 Protein, His Tag [orb1550811]
> 95% by SDS-PAGE.
Recombinant Human IL-3 Protein is produced by Human Cells expression system. The target protein is expressed with sequence (Ala20-Phe152) of human IL-3 (Accession #P08700) fused with a 6��His Tag at the C-terminus.
50 μg
