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Catalog Number | orb257621 |
---|---|
Category | Proteins |
Description | Human IL-18BP, His Tag is expressed from human 293 cells (HEK293). It contains AA Thr 31 - Gly 194 (Accession # NP_001138529). |
Target | IL-18BP |
Conjugation | Unconjugated |
Tag | C-6×His |
Reactivity | Human |
Form/Appearance | Powder |
Buffer/Preservatives | PBS, pH 7.4 |
Purity | 90% |
Protein Sequence | NP_001138529 |
UniProt ID | O95998 |
MW | 18.5 kDa |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 18.5 kDa. The protein migrates as 35-45 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expression System | HEK293 |
Endotoxins | 1.0 EU per μg |
Biological Origin | Human |
Expression Region | Thr 31 - Gly 194 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Note | For research use only |
NCBI | NP_001138529 |
SDS-PAGE analysis of Human IL-18BP protein
99.20% | |
19 kDa (predicted); 40.3 kDa (reducing condition, due to glycosylation) |
98.00% | |
20.5 kDa (predicted). Due to glycosylation, the protein migrates to 52-65 kDa based on Tris-Bis PAGE result. |
98.00% | |
21.14 kDa (predicted). Due to glycosylation, the protein migrates to 50-65 kDa based on Tris-Bis PAGE result. |
98.00% | |
21.14 kDa (predicted). Due to glycosylation, the protein migrates to 35-65 kDa based on Tris-Bis PAGE result. |