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Human IL-13 Protein, Fc Tag

Catalog Number: orb612113

DispatchUsually dispatched within 5-10 working days
$ 700.00
Catalog Numberorb612113
CategoryProteins
DescriptionHuman IL-13, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Gly 21 - Asn 132 (Accession # AAK53823.1).
TargetIL-13
ConjugationUnconjugated
TagC-Fc
ReactivityHuman
Form/AppearancePowder
Buffer/PreservativesTris with Glycine, Arginine and NaCl, pH 7.5
Purity90%
Protein SequenceAAK53823.1
MW38.8 kDa
Application notesThis protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 38.8 kDa. The protein migrates as 50-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Expression SystemHEK293
Endotoxins1.0 EU per μg
Biological OriginHuman
Biological ActivityLoaded Human IL-13, Fc Tag (Cat. No. ) on Protein A Biosensor, can bind Human IL-13 R alpha 2, His Tag (Cat. No. IL2-H52H5) with an affinity constant of 6.09 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Expression RegionGly 21 - Asn 132
Storage-20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
NoteFor research use only
NCBIAAK53823.1
Human IL-13 Protein, Fc Tag

Loaded Human IL-13, Fc Tag (Cat. No. orb612113) on Protein A Biosensor, can bind Human IL-13 R alpha 2, His Tag (Cat. No. orb612134) with an affinity constant of 6.09 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

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    62.7 kDa

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    The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.

    The protein has a predicted molecular mass of 38.4 kDa after removal of the signal peptide. The apparent molecular mass of IL13(35-146)-hFc is approximately 33-53 kDa due to glycosylation.

    Mammalian

    100 μg, 50 μg, 10 μg
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    The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.

    The protein has a predicted molecular mass of 38.1 kDa after removal of the signal peptide. The apparent molecular mass of IL13-hFc is approximately 35-70 kDa due to glycosylation.

    Mammalian

    100 μg, 10 μg, 50 μg
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