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Catalog Number | orb612113 |
---|---|
Category | Proteins |
Description | Human IL-13, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Gly 21 - Asn 132 (Accession # AAK53823.1). |
Target | IL-13 |
Conjugation | Unconjugated |
Tag | C-Fc |
Reactivity | Human |
Form/Appearance | Powder |
Buffer/Preservatives | Tris with Glycine, Arginine and NaCl, pH 7.5 |
Purity | 90% |
Protein Sequence | AAK53823.1 |
MW | 38.8 kDa |
Application notes | This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 38.8 kDa. The protein migrates as 50-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expression System | HEK293 |
Endotoxins | 1.0 EU per μg |
Biological Origin | Human |
Biological Activity | Loaded Human IL-13, Fc Tag (Cat. No. ) on Protein A Biosensor, can bind Human IL-13 R alpha 2, His Tag (Cat. No. IL2-H52H5) with an affinity constant of 6.09 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). |
Expression Region | Gly 21 - Asn 132 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Note | For research use only |
NCBI | AAK53823.1 |
Loaded Human IL-13, Fc Tag (Cat. No. orb612113) on Protein A Biosensor, can bind Human IL-13 R alpha 2, His Tag (Cat. No. orb612134) with an affinity constant of 6.09 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Unconjugated | |
95% | |
50.2 kDa & 63.1 kDa |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 38.4 kDa after removal of the signal peptide. The apparent molecular mass of IL13(35-146)-hFc is approximately 33-53 kDa due to glycosylation. | |
Mammalian |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 38.1 kDa after removal of the signal peptide. The apparent molecular mass of IL13-hFc is approximately 35-70 kDa due to glycosylation. | |
Mammalian |