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Catalog Number | orb1496180 |
---|---|
Category | Proteins |
Description | Human IL-10, premium grade is expressed from human 293 cells (HEK293). It contains AA Ser 19 - Asn 178 (Accession # P22301.1). |
Target | IL-10 |
Conjugation | Unconjugated |
Tag | Native |
Reactivity | Human |
Form/Appearance | Powder |
Buffer/Preservatives | PBS, pH 7.4 |
Purity | 95% |
Protein Sequence | NP_000563.1 |
MW | 18.6 kDa |
Application notes | This protein carries no "tag". The protein has a calculated MW of 18.6 kDa. The protein migrates as 17 kDa±3 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expression System | HEK293 |
Endotoxins | 0.01 EU per μg |
Biological Origin | Human |
Biological Activity | Immobilized Human IL-10, premium grade (Cat. No. ) at 5 μg/mL (100 μL/well) can bind Cynomolgus IL-10 R alpha Protein, Fc Tag (Cat. No. ILR-C5256) with a linear range of 0.1-2 ng/mL (QC tested). |
Expression Region | Ser 19 - Asn 178 |
Storage | -20°C to -70°C for 24 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Note | For research use only |
NCBI | NP_000563.1 |
Immobilized Human IL-10, premium grade (Cat. No. orb1496180) at 5 μg/mL (100 μL/well) can bind Cynomolgus IL-10 R alpha Protein, Fc Tag (Cat. No. orb1496248) with a linear range of 0.1-2 ng/mL (QC tested).
> 95% as determined by SDS-PAGE and HPLC. | |
18.6 kDa | |
E.Coli |
> 97% as determined by SDS-PAGE and HPLC. | |
11.2 kDa | |
E.Coli |
Greater than 95% as determined by SDS-PAGE. | |
16.9 kDa | |
E.coli |