You have no items in your shopping cart.
Cart summary
| Catalog Number | orb782369 |
|---|---|
| Category | Assays and Kits |
| Description | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Glycosylated Ferritin (GF). |
| Reactivity | Human |
| Concentration | 2000 nmol/mL |
| Tested applications | ELISA |
| Application notes | standard: 2000 nmol/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human GF protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GF in the samples is then determined by comparing the OD of the samples to the standard curve |
| Assay Type | Competitive |
| Assay Time | 2.5h |
| Range | 31.25-2000 nmol/mL |
| Sensitivity | 10.21 nmol/mL |
| Sample Types | serum, plasma |
| Alternative names | Glycosylated Ferritin ELISA Kit, GF ELISA Kit |
| Note | For research use only |
