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Human GDF-15 Protein, Fc-Avi Tag (Biotin)
Catalog Number: orb1184782
| Catalog Number | orb1184782 |
|---|---|
| Category | Proteins |
| Description | Biotinylated Human GDF-15, , Fc Tag is expressed from human 293 cells (HEK293). It contains AA Ala 197 - Ile 308 (Accession # Q99988-1). |
| Target | GDF-15 |
| Conjugation | Biotin |
| Tag | N-Avi & N-Fc |
| Reactivity | Human |
| Form/Appearance | Powder |
| Buffer/Preservatives | PBS, pH 7.4 |
| Purity | 90% |
| Protein Sequence | NP_004855.2 |
| UniProt ID | Q99988 |
| MW | 40.4 kDa |
| Application notes | This protein carries an Avi tag at the N-terminus, followed by a human IgG1 Fc tag. The protein has a calculated MW of 40.4 kDa. As a result of glycosylation, the protein migrates as 50-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition, and 80-90 kDa under non-reducing (NR) condition (SDS-PAGE). |
| Expression System | HEK293 |
| Endotoxins | 1.0 EU per μg |
| Biological Origin | Human |
| Biological Activity | Immobilized Human GFR alpha-like, His Tag (Cat. No. GFA-H52H3) at 5 μg/mL (100 μL/well) can bind Biotinylated Human GDF-15, , Fc Tag (Cat. No. ) with a linear range of 0.2-8 ng/mL (QC tested). |
| Expression Region | Ala 197 - Ile 308 |
| Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
| Note | For research use only |
| NCBI | NP_004855.2 |
Immobilized Human GFR alpha-like, His Tag (Cat. No. orb545746) at 5 μg/mL (100 μL/well) can bind Biotinylated Human GDF-15, Avitag, Fc Tag (Cat. No. orb1184782) with a linear range of 0.2-8 ng/mL (QC tested).
