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Human FGF basic Protein, His-Avi Tag (Biotin)
Catalog Number: orb752326
| Catalog Number | orb752326 |
|---|---|
| Category | Proteins |
| Description | FGF basic is a member of the FGF family of at least 23 related mitogenic proteins which show 35-60% amino acid conservation. FGF acidic and basic, unlike the other members of the family, lack signal peptides and are apparently secreted by mechanisms other than the classical protein secretion pathway. FGF basic has been isolated from a number of sources, including neural tissue, pituitary, adrenal cortex, corpus luteum, and placenta. This factor contains four cysteine residues, but reduced FGF basic retains full biological activity, indicating that disulfide bonds are not required for this activity. bFGF is a critical component of human embryonic stem cell culture medium; the growth factor is necessary for the cells to remain in an undifferentiated state, although the mechanisms by which it does this are poorly defined. It has been demonstrated to induce gremlin expression which in turn is known to inhibit the induction of differentiation by bone morphogenetic proteins. It is necessary in mouse-feeder cell dependent culture systems, as well as in feeder and serum-free culture systems. |
| Target | FGF basic |
| Conjugation | Biotin |
| Tag | N-Avi & N-10×His |
| Reactivity | Human |
| Form/Appearance | Powder |
| Buffer/Preservatives | 50 mM Tris, 150 mM NaCl, pH 7.5 |
| Purity | 90% |
| Protein Sequence | NP_001997.5 |
| UniProt ID | P09038 |
| MW | 20.1 kDa |
| Application notes | This protein carries an Avi tag at the N-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 20.1 kDa. The protein migrates as 20-22 kDa under reducing (R) condition (SDS-PAGE). |
| Expression System | E. coli |
| Endotoxins | 1.0 EU per μg |
| Biological Origin | Human |
| Expression Region | Pro 143 - Ser 288 |
| Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
| Note | For research use only |
| NCBI | NP_001997.5 |
