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| Catalog Number | orb334894 |
|---|---|
| Category | Proteins |
| Description | Human Dkk-1, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Thr 32 - His 266 (Accession # NP_036374.1). |
| Target | Dkk-1 |
| Conjugation | Unconjugated |
| Tag | C-Fc |
| Reactivity | Human |
| Form/Appearance | Powder |
| Buffer/Preservatives | PBS, pH 7.4 |
| Purity | 95% |
| UniProt ID | NP_036374.1 |
| MW | 52.4 kDa |
| Application notes | This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 52.4 kDa. The protein migrates as 67-85 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Expression System | HEK293 |
| Endotoxins | 1.0 EU per μg |
| Expression Region | Thr 32 - His 266 |
| Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
| Research Area | Disease Biomarkers |
| Note | For research use only |
| Expiration Date | 6 months from date of receipt. |

SDS-PAGE analysis of Human Dkk-1 protein
Unconjugated | |
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. | |
Predicted: 52.7 KDa. Observed: 60-80 KDa, reducing conditions |
The purity of the protein is greater than 90% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 51.9 kDa after removal of the signal peptide.The apparent molecular mass of DKK1-hFC is approximately 70 kDa due to glycosylation. | |
Mammalian |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 52.0 kDa after removal of the signal peptide. The apparent molecular mass of DKK1-mFc is approximately 55-70 kDa due to glycosylation. | |
E.coli |
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