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Catalog Number | orb545757 |
---|---|
Category | Proteins |
Description | Human CTGF, His Tag is expressed from human 293 cells (HEK293). It contains AA Gln 27 - Ala 349 (Accession # Q5M8T4-1). |
Target | CTGF |
Conjugation | Unconjugated |
Tag | C-10×His |
Reactivity | Human |
Form/Appearance | Powder |
Buffer/Preservatives | Tris with Potassium glutamate and Arginine, pH 7.0 |
Purity | 90% |
Protein Sequence | NP_001892.1 |
UniProt ID | Q5M8T4 |
MW | 37.4 kDa |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 37.4 kDa. The protein migrates as 36-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expression System | HEK293 |
Endotoxins | 1.0 EU per μg |
Biological Origin | Human |
Biological Activity | Loaded Monoclonal Anti-Human CTGF Antibody, Human IgG1 on AHC Biosensor, can bind Human CTGF, His Tag (Cat. No. ) with an affinity constant of 6.29 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). |
Expression Region | Gln 27 - Ala 349 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Note | For research use only |
NCBI | NP_001892.1 |
Loaded Monoclonal Anti-Human CTGF Antibody, Human IgG1 on AHC Biosensor, can bind Human CTGF, His Tag (Cat. No. orb545757) with an affinity constant of 6.29 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
> 95% by Tris-Bis PAGE, > 95% by SEC-HPLC | |
Recombinant Human CTGF / CCN2 Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Gln27-Ala349) of Human CTGF / CCN2 fused with a His Tag at the C-terminal. |
Greater than 95.0% as determined by SDS-PAGE. | |
HEK293 cells |
> 95% as determined by Bis-Tris PAGE | |
38.4 kDa (predicted). Due to glycosylation, the protein migrates to 43-53 kDa based on Tris-Bis PAGE result. |