Human CLDN18.2 full length protein-synthetic nanodisc

• Catalog Number: orb1291760
• Category: Proteins
• Description: Human CLDN18.2 full length protein-synthetic nanodisc
• Target: CLDN18.2
• Tag: C-Flag Tag
• Reactivity: Human
• Form/Appearance: Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. Do not use solvents with pH lower than 6.5 in subsequent experiments.
• UniProt ID: P56856
• MW: The human full length CLDN18.2 Protein has a MW of 27.5 kDa
• Expression System: HEK293
• Source: Mammalian
• Storage: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
• Alternative names: CLDN18.2
• Research Area: Protein Biochemistry
• Note: For research use only

Elisa plates were added with Flag Tag CLDN18.2-Nanodisc (0.2 μg/per well) on an anti-Flag monoclonal antibody pre-coated (0.2 μg/per well) plate. Serial diluted anti- CLDN18.2 monoclonal antibody (orb1173630) solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti- CLDN18.2 monoclonal antibody binding with CLDN18.2-Nanodisc is 593.6 ng/ml.

Human CLDN18.2-Nanodisc, Flag Tag on SDS-PAGE

Human CLDN18.2-Nanodisc can bind Anti-CLDN18.2 antibody (orb1173630) with an affinity constant of 1.619 nM as determined in a SPR assay.