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Human Cholecystokinin 18 (CCK18) ELISA Kit

Catalog Number: orb778737

DispatchUsually dispatched within 5-10 working days
$ 310.00
Catalog Numberorb778737
CategoryAssays and Kits
DescriptionThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cholecystokinin 18(CCK18) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cholecystokinin 18(CCK18).
ReactivityHuman
Concentration1000 pg/mL
Tested applicationsELISA
Application notesstandard: 1000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human CCK18 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CCK18. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CCK18 in the samples is then determined by comparing the OD of the samples to the standard curve
Assay TypeCompetitive
Assay Time2.5h
Range15.63-1000 pg/mL
Sensitivity4.28 pg/mL
Sample TypesSerum, plasma and other biological fluids.
Alternative namesCCK18
NoteFor research use only
Human Cholecystokinin 18 (CCK18) ELISA Kit