Human Butyric Acid (BA) ELISA Kit

• Catalog Number: orb782455
• Category: Assays and Kits
• Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Butyric Acid(BA) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Butyric Acid(BA).
• Reactivity: Human
• Concentration: 10000 pg/mL
• Tested applications: ELISA
• Application notes: standard: 10000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human BA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human BA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human BA in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Competitive
• Assay Time: 2.5h
• Range: 156.25-10000 pg/mL
• Sensitivity: 49.2 pg/mL
• Sample Types: Biological agents
• Alternative names: Propanecarboxylic Acid ELISA Kit, Butanoic Acid EL
• Note: For research use only