Human Beta-2-Microglobulin (BMG/b2-MG) ELISA Kit

• Catalog Number: orb775065
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Beta-2-Microglobulin(b2M).
• Reactivity: Human
• Concentration: 20 µg/mL
• UniProt ID: P61769
• Tested applications: ELISA
• Application notes: standard: 25 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human BMG/b2-MG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human BMG/b2-MG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human BMG/b2-MG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human BMG/b2-MG in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.4-25 ng/mL
• Sensitivity: 0.16 ng/mL
• Sample Types: serum, plasma, urine and other biological fluids
• Alternative names: Beta 2M,BMG ELISA Kit, B2MG ELISA Kit
• Research Area: Infectious Disease & Virology
• Note: For research use only
• Expiration Date: Please enquire.