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Catalog Number | orb257185 |
---|---|
Category | Proteins |
Description | Human Angiopoietin-2 (275-496), His Tag is expressed from human 293 cells (HEK293). It contains AA Lys 275 - Phe 496 (Accession # AAI26201.1). |
Target | Angiopoietin-2 |
Conjugation | Unconjugated |
Tag | N-6×His |
Reactivity | Human |
Form/Appearance | Powder |
Buffer/Preservatives | 20 mM MOPS, 150 mM NaCl, pH 7.5 |
Purity | 95% |
Protein Sequence | AAI26201.1 |
UniProt ID | O15123 |
MW | 26.3 kDa |
Application notes | This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 26.3 kDa. The protein migrates as 30-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expression System | HEK293 |
Endotoxins | 1.0 EU per μg |
Biological Origin | Human |
Expression Region | Lys 275 - Phe 496 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Note | For research use only |
NCBI | AAI26201.1 |
SDS-PAGE analysis of Human Angiopoietin-2 protein
> 95% as determined by SDS-PAGE |
> 92%, determined by SDS-PAGE | |
This protein contains the human Angiopoietin 2 (NP_001138.1) (Met 1-Phe 496) was expressed from HEK293 Cells. |
> 95%, determined by SDS-PAGE | |
This protein contains the human ANGPT2 (NP_001138.1) (Lys275-Phe496) was expressed with a polyhistidine tag at the N-terminus and expressed from HEK293 Cells. |