HSP90 Antibody

• Catalog Number: orb1822502
• Category: Antibodies
• Description: Mouse Anti-Water Mold HSP90 Monoclonal IgG2b
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: AC-16
• Tested applications: AM, IHC, WB
• Reactivity: Fungi, Gallus, Human, Insect, Invertebrate, Mouse, Plant, Rabbit, Rat
• Isotype: IgG2b
• Immunogen: Heat shock protein 90 from the water mold Achyla ambisexualis
• Concentration: 1 mg/ml
• Dilution range: WB (1:1000), IHC (1:2000); optimal dilutions for assays should be determined by the user.
• Conjugation: Unconjugated
• MW: 90kDa
• Target: HSP90
• Entrez: 4768
• UniProt ID: Q8LLI5
• NCBI: AAM90675.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated
• Alternative names: HSP84 Antibody, HSP86 Antibody, HSP90 Antibody, HS
• Note: For research use only
• Application notes: 1 µg/ml of SMC-112 was sufficient for detection of HSP90 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone AC-16. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:2000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 μl for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x. Inflammatory cells.

Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone AC-16. Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:2000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 μl for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x. Mostly inflammatory cells, some mucosa.