HSP25/HSP27 Antibody

• Catalog Number: orb1822500
• Category: Antibodies
• Description: Mouse Anti-Human HSP25/HSP27 Monoclonal IgG1 Kappa
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 8A7
• Tested applications: FACS, FC, ICC, IF, IHC, IP, WB
• Reactivity: Bovine, Canine, Guinea pig, Hamster, Human, Mouse, Rat, Sheep
• Isotype: IgG1 Kappa
• Immunogen: Human HSP27 peptide
• Concentration: 1 mg/ml
• Dilution range: WB (1:5000), ICC/IF (1:200), IHC (1:100); optimal dilutions for assays should be determined by the user.
• Conjugation: Unconjugated
• MW: 27kDa
• Target: HSP27
• Entrez: 3315
• UniProt ID: P04792
• NCBI: NP_001532.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated
• Alternative names: HSPB1 Antibody, Heat shock protein beta-1 Antibody
• Note: For research use only
• Application notes: A 1:5000 dilution of SMC-114 was sufficient for detection of HSP27 in 20 µg of HeLa cell lysate by ECL immunoblot analysis.
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry analysis using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 8A7. Tissue: embryo somites. Species: Rat. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody at 1:1000. Secondary Antibody: FITC Goat Anti-Mouse (green). Counterstain: Rhodamine-phalloidin labeled actin (red). Investigation at 11 days gestation.

Immunohistochemistry analysis using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 8A7. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody at 1:5000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 μl for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.

Immunohistochemistry analysis using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 8A7. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.

Immunohistochemistry analysis using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 8A7. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.

Western Blot analysis of Rat Lung tissue lysates showing detection of Hsp27 protein using Mouse Anti-Hsp27 Monoclonal Antibody, Clone 8A7. Load: 15 μg. Block: 5% blocking solution. Primary Antibody: Mouse Anti-Hsp27 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse: HRP for 1 hour at RT.