HLA-DQ Antibody (MHC II)

• Catalog Number: orb606900
• Category: Antibodies
• Description: Recognizes a DQ antigen, which is a dimer of 60kDa. The class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B Lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35kDa. It is encoded by 5 exons; exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation.
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: Rabbit IgG, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: Full length recombinant human protein was used as the immunogen for the recombinant HLA-DQ antibody.
• UniProt ID: P01920, P01906, P01908
• Tested applications: FACS, IF, IHC-Fr
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml for 30 min at RT
• Application notes: Optimal dilution of the recombinant HLA-DQ antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: HLA-DQA1/2866R
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Research Area: Epigenetics, Immunology
• Note: For research use only

Immunofluorescent staining of human Raji cells with recombinant HLA-DQ antibody (green, clone HLA-DQA1/2866R) and Reddot nuclear stain (red).

FACS testing of human Raji cells with recombinant HLA-DQ antibody (blue, clone HLA-DQA1/2866R) and isotype control (red).

SDS-PAGE analysis of purified, BSA-free recombinant HLA-DQ antibody (clone HLA-DQA1/2866R) as confirmation of integrity and purity.