GAG Antibody (Biotin)

• Catalog Number: orb1671407
• Category: Antibodies
• Description: GAG Antibody (Biotin)
• Target: GAG
• Clonality: Monoclonal
• Isotype: IgG2b
• Conjugation: Biotin
• Reactivity: Virus
• Form/Appearance: Liquid
• Concentration: 1 mg/mL
• Buffer/Preservatives: HIV-1 p24 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
• Purification: Anti-HIV-1 p24 antibody is immunoaffinity chromatography purified IgG.
• Immunogen: Mouse monoclonal HIV-1 p24 antibody was raised against a recombinant full-length HIV-1 p24 protein.
• UniProt ID: P04591
• MW: 24 kDa
• Tested applications: ELISA, WB
• Application notes: WB: 1 μg/mL. Anti-HIV-1 p24 antibody detects HIV-1 p24 protein by ELISA and WB. All other applications and species not yet tested.
• Antibody Type: Primary Antibody
• Clone Number: 8A1A6
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-HIV-1 p24 antibody: Pr55Gag, Gag polyprotein,
• Research Area: Infectious Disease & Virology
• Note: For research use only
• NCBI: AAB50258

Western Blot Validation with HIV-1 p24 Protein.Loading: 30 ng HIV-1 p24 recombinant protein. Antibodies: HIV-1 p24, orb1671408, 0.5 μg/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution.

ELISA Validation of HIV-1 p24 Antibody with HIV-1 p24 Protein.Coating Antigen: HIV-1 p24 recombinant protein, 1 μg/mL, incubated at 4°C overnight.Detection Antibodies: HIV-1 p24 antibody, orb1671408, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr.Secondary Antibodies: Goat anti-mouse HRP at 1:5000, incubated at RT for 1 hr.